Analysis Note

ControlHEK293 cell lysate

Application

Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected AES in HeLa cells.

Immunofluorescence Analysis: 20 µg/mL from a representative lot detected AES in HeLa cells.

Anti-AES Antibody is a highly specific rabbit polyclonal antibody, that targets Amino-terminal enhancer of split & has been tested in western blotting, ICC & Immunofluorescence.

Research Sub CategoryAdhesion (CAMs)

Research CategoryCell StructureCell Structure

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Amino-terminal enhancer of split (AES), also known as GRG or ESP1 or TLE5 or Gp130-associated protein GAM, is a member of the Groucho/ transducin-like enhancer of split (TLE) family. AES belongs to a group of transcriptional co-repressors that play an important role in neurogenesis, segmentation and sex determination. AES is suggested to be a dominant repressor towards other family members of the TLE family and might be required for the initiation and maintenance of the differentiated state. AES has been shown to inhibit the NF-kappa-B-regulated gene expression. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1). Recent studies suggest that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. The apoptosis of cells transfected with AES and Bit1 implies that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and more specifically – with the Alpha5Beta1 integrin. AES is found predominantly in muscle, heart and placenta. Additionally, in fetal tissues, AES is expressed highly in the heart, lung, kidney, brain and liver.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human AES.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected AES in 15 µg of HEK293 cell lysate.

Specificity

This antibody recognizes the C-terminus of AES.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~22 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 2 isoforms at ~22 kDa and ~29 kDa